Webinar Series
Tutorial 2: Measuring Bioenergetics in Primary Cell Types
August 27, 2008
11:00AM EDT/8:00AM PDT
Speaker:
David Ferrick, PhD
VP Biology & Applications Seahorse Bioscience, Inc.
Event Highlight
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Real-Time, Non-Invasive Measurement of Cellular Bioenergetics
Seahorse Bioscience's Extracellular Flux (XF) assays non-invasively profile the physiologic activity of mammalian cells in minutes, offering scientists a cell based assay for determination of in vitro oxygen consumption, mitochondrial function, fatty acid oxidation, uncoupling, toxicity, and ATP turnover.
XF Analyzer
XF technology is embodied in an easy-to-use instrument that takes little lab space, is microplate based, and does not disrupt existing cell culture procedures.
Specialty Labware
Whether you are looking for an experienced OEM partner to design and mold components for your new product or a scientist seeking a custom plate we have the expertise to help you.”
XF Training Course
During this 2-day, hands-on training class, students perform multiple assays as they learn about proper tissue culture techniques, the impact of culture conditions on cell physiology, and how the XF Analyzer can be leveraged in their research work.
XF Assay Kits - Disposable XF assay kits are essential to the operation of the XF24 Analyzer. Each assay kit consists of a disposable, 24-well sensor cartridge, a calibration plate, and a lid. Each dual-analyte sensor cartridge is designed to measure OCR (oxygen consumption rate) and ECAR (extracellular acidification rate) simultaneously.
Fatty Acid Oxidation - Extracellular Flux (XF) assays measure the two major energy producing pathways of the cell to profile fatty acid oxidation (FAO) without radioactivity. The result is less labor and cost per assay, greater throughput in less time, and an overall better view of metabolism with real-time, kinetic measurements.
Mitochondrial Function - Extracellular Flux (XF) assays allow you to measure mitochondrial function in live cells with less setup and clean up. By measuring the two major energy producing pathways of the cell simultaneously, scientist get the most physiologically relevant assay available. Now you can work with adherent cells and perform more experiments in less time. The result is a better overall view of metabolism.
